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KMID : 0811719980020050601
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Korean Journal of Physiology & Pharmacology
1998 Volume.2 No. 5 p.601 ~ p.609
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Role of Phospholipase A2 in Oxidant-induced Alteration in Phosphate Transport in Primary Cultured Rabbit Renal Proximal Tubule Cells
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Kwon Moo Park
Sun Hee Ko/Jae Suk Woo/Jin Sup Jung/Sang Ho Lee/Yong Keun Kim
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Abstract
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The present study was undertaken to examine the role of phospholipase A2(PLA2) in oxidant-induced inhibition of phosphate transport in primary cultured rabbit renal proximal tubule cells. Uptakes of phosphate and glucose were dose-dependently inhibited by an oxidant t-butylhydroperoxide (tBHP), and the significant inhibition appeared at 0.025 mM of tBHP, whereas tBHP-induced alterations in lipid peroxidation and cell viability were seen at 0.5 mM. tBHP stimulated arachidonic acid (AA) release in a dose-dependent fashion. A PLA2 inhibitor mepacrine prevented tBHP-induced AA release, but it did not alter the inhibition of phosphate uptake and the decrease in cell viability induced by tBHP. tBHP-induced inhibition of phosphate transport was not affected by a PKC inhibitor, staurosporine. tBHP at 0.1 mM did not produce the inhibition of Na+?K+?ATPase activity in microsomal fraction, although it significantly inhibited at 1.0 mM. These results suggest that tBHP can inhibit phosphate uptake through a mechanism independent of PLA2 activation, irreversible cell injury, and lipid peroxidation in primary cultured rabbit renal proximal tubular cells.
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KEYWORD
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Phosphate uptake, Oxidant, PLA2, Proximal tubular cells,
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